rmt4 54  (Bio X Cell)

Journal: Cell reports

Article Title: Tissue-resident, extravascular Ly6c − monocytes are critical for inflammation in the synovium

doi: 10.1016/j.celrep.2023.112513

Figure Lengend Snippet: (A) Pairwise Pearson’s correlation of global gene expression between replicates of NCMs, DCs, and TR-MCs. (B) Relative expression of representative genes from gene ontology (GO) processes associated with NCMs, DCs, and TR-MCs. (C) Clustering of RA patient CD45 + CD11b + synovial cells based on gene expression from CITE-seq. (D) ADT intensity of CD45, CD11b, and CD14 on RA patient synovial cells. (E and F) TR-MC module score in RA patient data. (G and H) Expression of Trem2 and Timd4 in RA patient data. (I–K) (I) ADT intensity of TIM4 in RA patient data. Immunofluorescence staining of synovial tissue from (J) healthy control and (K) RA patient.

Article Snippet: PE Tim4 RMT4-54 , BD Bioscience , 564147 RRID:AB_2738621.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Cell reports

Article Title: Tissue-resident, extravascular Ly6c − monocytes are critical for inflammation in the synovium

doi: 10.1016/j.celrep.2023.112513

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PE Tim4 RMT4-54 , BD Bioscience , 564147 RRID:AB_2738621.

Techniques: Recombinant, Purification, Liposomes, Isolation, Sequencing, Expressing, Software, Irradiation

Journal: Liver Research

Article Title: Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver ☆

doi: 10.1016/j.livres.2023.08.001

Figure Lengend Snippet: Key resources table.

Article Snippet: PerCP-eFluorTM 710 rat anti-mouse Tim4 (clone 54 (RMT4-54)) , Thermo Fisher Scientific , 46-5866-82 , 2294211 , .

Techniques: Recombinant, Blocking Assay, Staining, Software, Flow Cytometry

Journal: Liver Research

Article Title: Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver ☆

doi: 10.1016/j.livres.2023.08.001

Figure Lengend Snippet: Materials and equipment: extracellular antibody master mix.

Article Snippet: PerCP-eFluorTM 710 rat anti-mouse Tim4 (clone 54 (RMT4-54)) , Thermo Fisher Scientific , 46-5866-82 , 2294211 , .

Techniques: Blocking Assay, Staining, Flow Cytometry

Journal: Liver Research

Article Title: Optimization of high throughput spectral flow cytometry for immune cell profiling in mouse liver ☆

doi: 10.1016/j.livres.2023.08.001

Figure Lengend Snippet: Flow cytometry macrophage gating strategy. Gating strategy for identification of monocyte-derived macrophages (Mo-Macs) and Kupffer cells (KCs). Panel A continues from a CD45-positive gate from Fig. 1 D and gates on F4/80-positive cells. CD11b-positive cells are selected (B), and KCs and Mo-Macs are differentiated based on Clec2 and Tim4 expression (C). Panels D and F investigate interleukin (IL)-10 expression while panels E and G show CD206 expression for Mo-Macs and KCs, respectively. KCs are defined as CD45 + F4/80 + CD11b + and Clec2 and Tim4 dual positive. Mo-Macs are CD45 + F4/80 + CD11b + and not Clec2 and Tim4 dual positive. Mo-Macs are split from KCs by utilizing a “Not-Gate”, including all cells that are not gated as Clec2 and Tim4 dual positive in Fig1C.

Article Snippet: PerCP-eFluorTM 710 rat anti-mouse Tim4 (clone 54 (RMT4-54)) , Thermo Fisher Scientific , 46-5866-82 , 2294211 , .

Techniques: Flow Cytometry, Derivative Assay, Expressing

Journal: Cells

Article Title: Inflammatory Cell Dynamics after Murine Femoral Artery Wire Injury: A Multi-Parameter Flow Cytometry-Based Analysis

doi: 10.3390/cells12050689

Figure Lengend Snippet: Antibody staining panel and optimal staining concentration for approximately 1 × 10 5 cells in 50 μL volume.

Article Snippet: PE , Tim4 , Macrophage subsets , RMT4-54 , 2.5 , BD Biosciences.

Techniques: Staining, Concentration Assay, Marker

Journal: eLife

Article Title: FGF21 protects against hepatic lipotoxicity and macrophage activation to attenuate fibrogenesis in nonalcoholic steatohepatitis

doi: 10.7554/eLife.83075

Figure Lengend Snippet: List of antibodies and other reagents used for flow cytometry analyses.

Article Snippet: TIM4 , 54 (RMT4-54) , PerCP-eFluor710 , Thermo Fisher , 46-5866-82.

Techniques: Flow Cytometry, Staining